Hello, Valida-- and welcome to English Forums. 2. -- Because you have specified 'in this garden', the definite article is needed.
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valida1.Roses in this garden are beautiful.You can use either one.
2.The roses in this garden are beautiful.
validaDo you think the first sentence can refer to some of the roses in this garden while the second refers to all the roses in this garden?I don't get that impression at all. You have to use a quantifier or qualifying adjective if you don't mean all of them.
valida3. Wines of France are among the best in the world.It is arguable that any of these are OK.
4. The wines of France are among the best in the world.
5. Wines that France produces are among the best in the world.
6. The wines that France produces are among the best in the world.