0
NL888 Posted 13 years ago
Grammar
What does "flap" mean here?
Context:
More:
FEN Biology - What are flap endonucleases?
Flap endonucleases, 5' nucleases and 5'-3' exonucleases are some of the names applied to a group of ubiquitous structure-specific nucleases that can cleave branched DNA. They occur in all living organisms from bacteria to Homo sapiens. Some viruses even carry genes encoding their own FEN enzymes. In the last few years the weight of scientific papers in which these enzymes are called flap endonucleases (FENs) has increased so that is what we will call them here. Apart from being essential for all cells (they participate in DNA replication and repair processes), they are also widely used in biotechnology in genotyping, quantitative PCR, polymorphism screening and molecular biology. DNA polymerase I possesses a flap endonuclease domain in addition to the well known Klenow (or large) fragment carrying the polymerase and proofreading polymerase domains. http://www.sayers.staff.shef.ac.uk/fen/#FENs_IN_BIOTECH |
More:
Top answer
From Wikipedia, The endonuclease activity of FENs was initially identified as acting on a DNA duplex which has a single stranded 5' overhang on one of the strands (termed a "5' flap", hence the name flap endonuclease). org/wiki/Flap_endonuclease
- From Wikipedia, The endonuclease activity of FENs was initially identified as acting on a DNA duplex which has a single stranded 5' overhang on one of the strands (termed a "5' flap", hence the name flap endonuclease).
- org/wiki/Flap_endonuclease
Get the Weekly English Kit 📬
New words, one handy idiom, and a 2-minute quiz — delivered to your inbox to keep your streak alive.
7 Answers
0
From Wikipedia,
The endonuclease activity of FENs was initially identified as acting on a DNA duplex which has a single stranded 5' overhang on one of the strands (termed a "5' flap", hence the name flap endonuclease).
http://en.wikipedia.org/wiki/Flap_endonuclease
The endonuclease activity of FENs was initially identified as acting on a DNA duplex which has a single stranded 5' overhang on one of the strands (termed a "5' flap", hence the name flap endonuclease).
http://en.wikipedia.org/wiki/Flap_endonuclease
00
0
It means a single stranded 5' overhang on one of the strands, which presumably looks like an ordinary flap under a microscope.
00
0
In a sort of very specialized way I suppose it does, since it indicates the position of a carbon within the DNA backbone. All DNA strands terminate one end with a phosphate group (called the 5' end) and one end with a hydroxyl group (the 3' end). The numbers correspond to where the groups bond to the carbon ring of the sugar.
Let me caution you that trying to learn English from scientifi
Let me caution you that trying to learn English from scientifi
00
"flap" means overhang.
...........................| - - flap - - |
-------------------------------------
-----------------------
The two lines represent two strands of DNA. One is longer than the other. The longer one has a flap. When the two strands combine (anneal), there will be DNA left over in one strand which cannot pair to anything on the other strand. This is
...........................| - - flap - - |
-------------------------------------
-----------------------
The two lines represent two strands of DNA. One is longer than the other. The longer one has a flap. When the two strands combine (anneal), there will be DNA left over in one strand which cannot pair to anything on the other strand. This is
00
NL888 Does 5' mean number fifth?5' is the fifth carbon atom in the ribose that is part of the backbone of DNA.
A phosphate connects carbon atom 3' on one ribose to carbon atom 5' on another.
(Actually, deoxyribose, but the numbering is the same for both.)
There's a diagram of it here that shows the numbering of the 5 carbons.
0
Related Questions