urgent help

Question

Please help me for my essay writing. I am not native speaker in English. I completed master degree in Genetics. I am in process of application for a PhD position. I found this web page very late. I look forward your replies quickly. Thank you for your concern

1. Please give your reasons for wishing to do a doctoral thesis in the field you have chosen and explain your longer-term professional goals. Prepare your statement of objectives in whatever form clearly presents your views. The Admission Committee will welcome any factors you wish to bring to its attention concerning your academic and work experience to date, as well as your objectives. (Keep a copy for your file. You may also wish to provide copies to your referees before they complete their forms.)(1 page max.)

I found that an international PhD position at University of XXX corresponds well with my future plans in seeking a career in the academic world, as a geneticist. I believe that dissertation of PhD is one of the important milestones in the career of a young scientist. Consequently, I want to do my doctorate thesis with conscious guide in a scientific environment.

Today, a number of people aim to study science. I am one of those who not only aim to achieve scientific professional understanding but also to use science as a tool for conveying the knowledge of science to humanity. This tool will surely help me to contribute a number of things to people and their lives. No education is complete without experience. Armed with a solid understanding of academic principles, I can continue my career where I will add to my experience. Because I will be well versed in the theory, I will be able to adapt and modify the theoretical constructs as required in order to meet practical researches.

It is my life ambition to do scientific research because I believe that scientific research and its methods provide an unrivalled framework in which the problems of the physical world can be determined and solutions to them be found. Solutions from “the laboratories” do not always translate into the real world completely, but scientific researches contribute a lot of things which have gone to achieve right solutions and then they are implementation. Pursuing the methods and seeing problems as important as acquiring positive conclusions. In addition, discovering the implied meanings behind the scientific knowledges give me pleasure and encourage me to think and research the subject matter.

I am not one of those who constantly study on a subject alone. Moreover, I am team spirited, effective communication, leadership skills, organizing and so on. In many team works or groups, I could lead the team well, and fight for the goal. Friends under different cultural backgrounds from various countries in international programme can be in my favor. I try to live a sociable life with my friends as well. Conducting such a graduate study, I firstly aim to contribute something to myself and then share it with people around. I like people who do not discriminate others according to race, nationality, sex, or religion. Therefore, I will try to support people who do not know absolutely how to solve their health problems through my studies in genetics. My future goals include establishing a career in research and educating people about science. As far as I know, scientific research has its origins in a very fundamental human character namely curiosity. I believe that curiosity creates questions in a scientist’s mind thus researches start. My curiosity and strong desire encourage me to be a scientist. It is very important, though, to ask the right question. During my four-year study at the university and then master degree, I found the answer to this question in the studies I conducted at the Molecular Biology Department at XXX University, Pediatric Molecular Genetic Department at the XXX University School of Medicine and I would like to be one of those who work incessantly to find out the answers. In my future career when I find questions and related right answers, I think I will have a great opportunity to continue my studies. Therefore, I am applying for your Doctoral Programme in Genetics starting February XX, 2006.

As a committed, thoughtful and hard-working person with my personality, practical and theoretical background, I believe that I am a good candidate for the PhD Program in the field of Genetics.

Savvysavz · Accepted Answer

Hi Whiteleaf,

Your essay does not say much about what you have done during your Masters. In fact it almost says nothing about the academic work you did in the past. You only talk about how science will benefit from you and how pleased you are but you need to prove it by mentioning about some research you did with a professor, or some journal you wrote, something to actually prove your academic past. The lines below clearly mention that:

The Admission Committee will welcome any factors you wish to bring to its attention concerning your academic and work experience to date

Do you still have time to re-write your essay? I or someone else will have no problem correcting this version for you but I'm saying this only for your benefit. No offence meant.

Savvy

Whiteleaf · Answer

Hi Savvysavz,

Thank u very much for your quick reply. When I read your comment, I thought your opinion. That is true. You are absolutely right. I changed first paragraph and added master subject. But I wait to reply journal which I wrote with my professor.

I still have time to re-write my essay. Thanks in advance.

Whiteleaf

1. Please give your reasons for wishing to do a doctoral thesis in the field you have chosen and explain your longer-term professional goals. Prepare your statement of objectives in whatever form clearly presents your views. The Admission Committee will welcome any factors you wish to bring to its attention concerning your academic and work experience to date, as well as your objectives. (Keep a copy for your file. You may also wish to provide copies to your referees before they complete their forms.)(1 page max.)

I found that an international PhD position at ISREC-EPFL corresponds well with my future plans in seeking a career in the academic world, as a geneticist. I believe that dissertation of PhD is one of the important milestones in the career of a young scientist. First milestone is my master thesis which subject is “Screening of the mitochondrial 12S rRNA (MTRNR1) gene in patients with sensorineural hearing loss”. In my master study I screened a hundred individuals by PCR-RFLP and PCR-TTGE techniques, for 1555 A>G , and other mutations in the MTRNR1 gene. Consequently, I want to do my doctorate thesis with conscious guide in a scientific environment.

Today, a number of people aim to study science. I am one of those who not only aim to achieve scientific professional understanding but also to use science as a tool for conveying the knowledge of science to humanity. This tool will surely help me to contribute a number of things to people and their lives. No education is complete without experience. Armed with a solid understanding of academic principles, I can continue my career where I will add to my experience. Because I will be well versed in the theory, I will be able to adapt and modify the theoretical constructs as required in order to meet practical researches.

It is my life ambition to do scientific research because I believe that scientific research and its methods provide an unrivalled framework in which the problems of the physical world can be determined and solutions to them be found. Solutions from “the laboratories” do not always translate into the real world completely, but scientific researches contribute a lot of things which have gone to achieve right solutions and then they are implementation. Pursuing the methods and seeing problems as important as acquiring positive conclusions. In addition, discovering the implied meanings behind the scientific knowledges give me pleasure and encourage me to think and research the subject matter.

I am not one of those who constantly study on a subject alone. Moreover, I am team spirited, effective communication, leadership skills, organizing and so on. In many team works or groups, I could lead the team well, and fight for the goal. Friends under different cultural backgrounds from various countries in international programme can be in my favor. I try to live a sociable life with my friends as well. Conducting such a graduate study, I firstly aim to contribute something to myself and then share it with people around. I like people who do not discriminate others according to race, nationality, ***, or religion. Therefore, I will try to support people who do not know absolutely how to solve their health problems through my studies in genetics. My future goals include establishing a career in research and educating people about science. As far as I know, scientific research has its origins in a very fundamental human character namely curiosity. I believe that curiosity creates questions in a scientist’s mind thus researches start. My curiosity and strong desire encourage me to be a scientist. It is very important, though, to ask the right question. During my four-year study at the university and then master degree, I found the answer to this question in the studies I conducted at the Molecular Biology Department at Hacettepe University, Pediatric Molecular Genetic Department at the Ankara University School of Medicine and I would like to be one of those who work incessantly to find out the answers. In my future career when I find questions and related right answers, I think I will have a great opportunity to continue my studies. Therefore, I am applying for your Doctoral Programme in Genetics starting February XX, 2006.

As a committed, thoughtful and hard-working person with my personality, practical and theoretical background, I believe that I am a good candidate for the PhD Program in the field of Genetics.

Savvysavz · Answer

Hi Whiteleaf,

I'm glad you made the changes

. Here's my correction in blue. You will find my comments/ suggestions in red.The sentence in green doesn't make much sense. If you really want it, I will alter it for you. Meanwhile if you still have time, I suggest you add more of what I'm asking from my comments in red. You still need to add more of your thesis and also, if you got some good marks you can add it in your essay.

Best of luck!
Savvy

_________________________________________________________

I believe that an international PhD position at ISREC-EPFL(expand ISREC-EPFL)corresponds well with my future plans in seeking a distinguished career in the eminent field of genetics. (please delete I believe that) A dissertation in PhD is (delete one of the) provides the cutting edge in the career of a young scientist.(delete First milestone is my master thesis which subject is) I chose “Screening of the mitochondrial 12S rRNA (MTRNR1) gene in patients with sensorineural hearing loss” for my Masters.During my thesis, I screened hundred individuals by PCR-RFLP and PCR-TTGE techniques, for 1555 A>G , and other mutations(name 1 or 2 other mutations)(delete the other in)of the MTRNR1 gene.(How about writing a brief result of your screening? Or some conclusion you derived?)(delete this sentence Consequently, I want to do my doctorate thesis with conscious guide in a scientific environment.)

Today, a number of people aim to study science. I am one of those who not only aim to achieve a professional scientific understanding but also to use science as a tool in propagating this knowledge (delete of science) for the benefit of humanity. Science will surely help me to contribute a number of things to people and their lives. No education is complete without experience. Armed with a solid academic background, I can continue my career in PhD which will add to my experience.Since I am already well-versed in theory, I will be able to adapt and modify the theoretical constructs as and when required in order to meet higher practical researches.

It is my life ambition to do scientific research because I believe that scientific research and its methods provide an unrivalled framework in which the problems of the physical world can be determined and solutions to them be found. Solutions from “the laboratories” do not always translate into the real world completely, but scientific researches contribute a lot of things which have gone to achieve right solutions and then they are implementation. Pursuing the methods and seeing problems as important as acquiring positive conclusions. In addition, discovering the implied meanings behind the scientific knowledges give me pleasure and encourage me to think and research the subject matter. (This paragraph in red is unnecessary...because it's almost a repetition of the previous paragaraph.)

I am not one who constantly studies on a subject alone.(delete Moreover,) I am team spirited and can communicate effectively. I hold strong leadership skills and I am good at organizing(add something to organizing). In many team works or groups, I could lead the team well, and persist till I reach my desired goal. Colleagues from multi-cultural backgrounds from various countries in an international atmosphere can be in my favor. I hold a good social circle of friends as well. Conducting such a graduate study, I first aim to acquire something for myself and then share it with people around me. I like people who do not discriminate others against race, nationality, ***, or religion. (delete Therefore, I will) I help ailing people (who do not know absolutely) by trying to solve their health problems through my studies in genetics. My future goals include establishing a career in research and educating people about science. As far as history goes (delete I know), scientific research originates from a very fundamental human character namely curiosity. I believe that curiosity creates questions in a scientist’s mind and from there researches start. My curiosity and strong ambition encouraged me to become a scientist. It is very important, though, to ask the right questions. During my four-year tenure at the university (delete and then) followed by a Masters degree, I found the answer to this question(write the question and the answer you discoverd from your research) in the studies I conducted at the Molecular Biology Department at Hacettepe University, Pediatric Molecular Genetic Department at the Ankara University School of Medicine and I would like to be one of those who work incessantly to find out more answers. In (delete my future career) the future, when I find questions and related right answers, I think I will have a great opportunity to continue my studies. (delete Therefore,) Keeping the above in mind, I am now applying for your Doctoral Programme in Genetics starting February ***, 2006.

As a committed, thoughtful and hard-working person with a dynamic personality, practical and theoretical background, I believe that I make a good candidate for the PhD Program in the field of Genetics.

Anonymous · Answer

Hi Savvy,

I have some time. Because I wait diploma of my high school which is wanted by uni for application. When I receive diploma from my parents in abroad, I complete then apply uni for PhD position.

I changed your correction. Please, check it. I will be very glad. And I want to help you another writing. Could you help me?

Thanks in advance!!

Whiteleaf

I believe that an international PhD position at the Swiss Institute for Experimental Cancer Research-école Polytechnique Fédérale de Lausanne (ISREC-EPFL) corresponds well with my future plans in seeking a distinguished career in the eminent field of genetics. A dissertation in PhD is provides the cutting edge in the career of a young scientist. I chose “Screening of the mitochondrial 12S rRNA (MTRNR1) gene in patients with sensorineural hearing loss” for my Masters. During my thesis, I screened hundred individuals by PCR-RFLP and PCR-TTGE techniques, for 1555 A>G, and other mutations (I aim to find this mutation so I did not write their names) of the MTRNR1 gene.

Today, a number of people aim to study science. I am one of those who not only aim to achieve a professional scientific understanding but also to use science as a tool in propagating this knowledge for the benefit of humanity. Science will surely help me to contribute a number of things to people and their lives. No education is complete without experience. Armed with a solid academic background, I can continue my career in PhD which will add to my experience. Since I am already well-versed in theory, I will be able to adapt and modify the theoretical constructs as and when required in order to meet higher practical researches. I was placed as the 3^rd among 98 students who were graduated from the same department in the same academic year (2002-2003).

In addition, discovering the implied meanings behind the scientific knowledges give me pleasure and encourage me to think and research the subject matter.

I am not one who constantly studies on a subject alone. I am team spirited and can communicate effectively. I hold strong leadership skills and I am good at organizing and analytical thinking. In many team works or groups, I could lead the team well, and persist till I reach my desired goal. Colleagues from multi-cultural backgrounds from various countries in an international atmosphere can be in my favour. I hold a good social circle of friends as well. Conducting such a graduate study, I first aim to acquire something for myself and then share it with people around me. I like people who do not discriminate others against race, nationality, ***, or religion. I help ailing people (who do not know absolutely) by trying to solve their health problems through my studies in genetics. My future goals include establishing a career in research and educating people about science. As far as history goes, scientific research originates from a very fundamental human character namely curiosity. I believe that curiosity creates questions in a scientist’s mind and from there researches start. My curiosity and strong ambition encouraged me to become a scientist. It is very important, though, to ask the right questions. During my four-year tenure at the university followed by a Masters degree, I found the answer to this question (Which mutations do induce hearing loss in 12S rRNA (MTRNR1) gene and what are other changes associated with aminoglycoside ototoxicity? The 1555 A>G mutation was found by PCR-RFLP in one patient with a history of aminoglycoside exposure. Two patients with no history of aminoglycoside exposure were found to have the 750 A>G polymorphism by the PCR-TTGE technique. No pathogenic base substitution was found) in the studies I conducted at the Molecular Biology Department at Hacettepe University, Pediatric Molecular Genetic Department at the Ankara University School of Medicine and I would like to be one of those who work incessantly to find out more answers. In the future, when I find questions` (which I create in my mind) right answers, I think I will have a great opportunity to continue my studies. Keeping the above in mind, I am now applying for your Doctoral Programme in Genetics starting February ***, 2006.

As a committed, thoughtful and hard-working person with a dynamic personality, practical and theoretical background, I believe that I make a good candidate for the PhD Program in the field of Genetics.

Note: I enclosure my master thesis abstract.

Anonymous · Answer

Hi Savvy,

I changed sntences which u correct. Please check it for me. I add some sentences in orange colour. And I want to write green sentences. If u modify this sentences and do correction,comments, I am very glad. I want to some help u for another writing. Could u help me for this?

I enclosure abstract of my master thesis.

I believe that an international PhD position at the Swiss Institute for Experimental Cancer Research-école Polytechnique Fédérale de Lausanne (ISREC-EPFL) corresponds well with my future plans in seeking a distinguished career in the eminent field of genetics. A dissertation in PhD is provides the cutting edge in the career of a young scientist. I chose “Screening of the mitochondrial 12S rRNA (MTRNR1) gene in patients with sensorineural hearing loss” for my Masters. During my thesis, I screened hundred individuals by PCR-RFLP and PCR-TTGE techniques, for 1555 A>G, and other mutations (I aim to find this mutation so I did not write their names) of the MTRNR1 gene.

Today, a number of people aim to study science. I am one of those who not only aim to achieve a professional scientific understanding but also to use science as a tool in propagating this knowledge for the benefit of humanity. Science will surely help me to contribute a number of things to people and their lives. No education is complete without experience. Armed with a solid academic background, I can continue my career in PhD which will add to my experience. Since I am already well-versed in theory, I will be able to adapt and modify the theoretical constructs as and when required in order to meet higher practical researches. I was placed as the 3^rd among 98 students who were graduated from the same department in the same academic year (2002-2003).

In addition, discovering the implied meanings behind the scientific knowledges give me pleasure and encourage me to think and research the subject matter.

I am not one who constantly studies on a subject alone. I am team spirited and can communicate effectively. I hold strong leadership skills and I am good at organizing and analytical thinking. In many team works or groups, I could lead the team well, and persist till I reach my desired goal. Colleagues from multi-cultural backgrounds from various countries in an international atmosphere can be in my favour. I hold a good social circle of friends as well. Conducting such a graduate study, I first aim to acquire something for myself and then share it with people around me. I like people who do not discriminate others against race, nationality, ***, or religion. I help ailing people (who do not know absolutely) by trying to solve their health problems through my studies in genetics. My future goals include establishing a career in research and educating people about science. As far as history goes, scientific research originates from a very fundamental human character namely curiosity. I believe that curiosity creates questions in a scientist’s mind and from there researches start. My curiosity and strong ambition encouraged me to become a scientist. It is very important, though, to ask the right questions. During my four-year tenure at the university followed by a Masters degree, I found the answer to this question (Which mutations do induce hearing loss in 12S rRNA (MTRNR1) gene and what are other changes associated with aminoglycoside ototoxicity? The 1555 A>G mutation was found by PCR-RFLP in one patient with a history of aminoglycoside exposure. Two patients with no history of aminoglycoside exposure were found to have the 750 A>G polymorphism by the PCR-TTGE technique. No pathogenic base substitution was found) in the studies I conducted at the Molecular Biology Department at Hacettepe University, Pediatric Molecular Genetic Department at the Ankara University School of Medicine and I would like to be one of those who work incessantly to find out more answers. In the future, when I find questions` (which I create in my mind) right answers, I think I will have a great opportunity to continue my studies. Keeping the above in mind, I am now applying for your Doctoral Programme in Genetics starting February ***, 2006.

As a committed, thoughtful and hard-working person with a dynamic personality, practical and theoretical background, I believe that I make a good candidate for the PhD Program in the field of Genetics.

Note: I enclosure my master thesis abstract.

ScreenIng of the MItochondrIal 12S rRNA (MTRNR1) Gene In PatIents wIth SensorIneural HearIng Loss

ABSTRACT

DNA damage with accumulation of reactive oxygen species results from mutations causing reduction of ATP synthesis in mitochondria in which the steps of oxidative phosphorylation occur at the final stage of cellular respiration in aerobic organisms. in many tissue and organ systems this insufficiency results in disorders. Some mutations affect mitochondrial translation, decreasing ATP production in the cochlea of the inner ear. A decreasing amount of ATP affects ion pumping negatively, damaging ion balance, which is very important in hearing function. Identified mutations, which are responsible for nonsyndromic hearing impairment in the mitochondrial DNA occur mostly in the MTRNR1 gene.

Mutations in 12S rRNA, which are maternally transmitted frequently cause hearing loss. They usually present in small families or as sporadic occurrances. One of the most common non-syndromic mutations in this gene is 1555 A>G. This mutation alone can induce hearing loss but it is also the most common change associated with aminoglycoside-induced deafness. Another mutation (delT961Cins(n)) in the 12S rRNA gene has also been identified, associated with aminoglycoside ototoxicity. Pathogenicity of this mutation has remained questionable. A number of other changes in this gene have been implied to be associated with hearing loss.

In this study, 70 individuals with hearing impairment, who do not have mutations in the GJB2 gene, were screened by PCR-RFLP and PCR-TTGE techniques, for 1555 A>G, and other mutations in the MTRNR1 gene. Individuals with hearing loss were coming from small families with other affecteds or were sporadic cases. Eleven patients had a history of aminoglycoside exposure. Thirty control individuals with no hearing impairment, and negative family history for deafness were also induced.

The 1555 A>G mutation was found by PCR-RFLP in one patient with a history of aminoglycoside exposure. Two patients with no history of aminoglycoside exposure were found to have the 750 A>G polymorphism by the PCR-TTGE technique. No pathogenic base substitution was found.

Key Words: MTRNR1, mtDNA mutation, TTGE, aminoglycoside ototoxicity, mitochondrial nonsyndromic deafness,

Whiteleaf · Answer

Hi Savvy,

I changed sntences which u correct. Please check it for me. I add some sentences in orange colour. And I want to write green sentences. If u modify this sentences and do correction,comments, I am very glad. I want to some help u for another writing. Could u help me for this?

I enclosure abstract of my master thesis.

I believe that an international PhD position at the Swiss Institute for Experimental Cancer Research-école Polytechnique Fédérale de Lausanne (ISREC-EPFL) corresponds well with my future plans in seeking a distinguished career in the eminent field of genetics. A dissertation in PhD is provides the cutting edge in the career of a young scientist. I chose “Screening of the mitochondrial 12S rRNA (MTRNR1) gene in patients with sensorineural hearing loss” for my Masters. During my thesis, I screened hundred individuals by PCR-RFLP and PCR-TTGE techniques, for 1555 A>G, and other mutations (I aim to find this mutation so I did not write their names) of the MTRNR1 gene.

Today, a number of people aim to study science. I am one of those who not only aim to achieve a professional scientific understanding but also to use science as a tool in propagating this knowledge for the benefit of humanity. Science will surely help me to contribute a number of things to people and their lives. No education is complete without experience. Armed with a solid academic background, I can continue my career in PhD which will add to my experience. Since I am already well-versed in theory, I will be able to adapt and modify the theoretical constructs as and when required in order to meet higher practical researches. I was placed as the 3^rd among 98 students who were graduated from the same department in the same academic year (2002-2003).

In addition, discovering the implied meanings behind the scientific knowledges give me pleasure and encourage me to think and research the subject matter.

I am not one who constantly studies on a subject alone. I am team spirited and can communicate effectively. I hold strong leadership skills and I am good at organizing and analytical thinking. In many team works or groups, I could lead the team well, and persist till I reach my desired goal. Colleagues from multi-cultural backgrounds from various countries in an international atmosphere can be in my favour. I hold a good social circle of friends as well. Conducting such a graduate study, I first aim to acquire something for myself and then share it with people around me. I like people who do not discriminate others against race, nationality, ***, or religion. I help ailing people (who do not know absolutely) by trying to solve their health problems through my studies in genetics. My future goals include establishing a career in research and educating people about science. As far as history goes, scientific research originates from a very fundamental human character namely curiosity. I believe that curiosity creates questions in a scientist’s mind and from there researches start. My curiosity and strong ambition encouraged me to become a scientist. It is very important, though, to ask the right questions. During my four-year tenure at the university followed by a Masters degree, I found the answer to this question (Which mutations do induce hearing loss in 12S rRNA (MTRNR1) gene and what are other changes associated with aminoglycoside ototoxicity? The 1555 A>G mutation was found by PCR-RFLP in one patient with a history of aminoglycoside exposure. Two patients with no history of aminoglycoside exposure were found to have the 750 A>G polymorphism by the PCR-TTGE technique. No pathogenic base substitution was found) in the studies I conducted at the Molecular Biology Department at Hacettepe University, Pediatric Molecular Genetic Department at the Ankara University School of Medicine and I would like to be one of those who work incessantly to find out more answers. In the future, when I find questions` (which I create in my mind) right answers, I think I will have a great opportunity to continue my studies. Keeping the above in mind, I am now applying for your Doctoral Programme in Genetics starting February ***, 2006.

As a committed, thoughtful and hard-working person with a dynamic personality, practical and theoretical background, I believe that I make a good candidate for the PhD Program in the field of Genetics.

Note: I enclosure my master thesis abstract.

ScreenIng of the MItochondrIal 12S rRNA (MTRNR1) Gene In PatIents wIth SensorIneural HearIng Loss

ABSTRACT

DNA damage with accumulation of reactive oxygen species results from mutations causing reduction of ATP synthesis in mitochondria in which the steps of oxidative phosphorylation occur at the final stage of cellular respiration in aerobic organisms. in many tissue and organ systems this insufficiency results in disorders. Some mutations affect mitochondrial translation, decreasing ATP production in the cochlea of the inner ear. A decreasing amount of ATP affects ion pumping negatively, damaging ion balance, which is very important in hearing function. Identified mutations, which are responsible for nonsyndromic hearing impairment in the mitochondrial DNA occur mostly in the MTRNR1 gene.

Mutations in 12S rRNA, which are maternally transmitted frequently cause hearing loss. They usually present in small families or as sporadic occurrances. One of the most common non-syndromic mutations in this gene is 1555 A>G. This mutation alone can induce hearing loss but it is also the most common change associated with aminoglycoside-induced deafness. Another mutation (delT961Cins(n)) in the 12S rRNA gene has also been identified, associated with aminoglycoside ototoxicity. Pathogenicity of this mutation has remained questionable. A number of other changes in this gene have been implied to be associated with hearing loss.

In this study, 70 individuals with hearing impairment, who do not have mutations in the GJB2 gene, were screened by PCR-RFLP and PCR-TTGE techniques, for 1555 A>G, and other mutations in the MTRNR1 gene. Individuals with hearing loss were coming from small families with other affecteds or were sporadic cases. Eleven patients had a history of aminoglycoside exposure. Thirty control individuals with no hearing impairment, and negative family history for deafness were also induced.

The 1555 A>G mutation was found by PCR-RFLP in one patient with a history of aminoglycoside exposure. Two patients with no history of aminoglycoside exposure were found to have the 750 A>G polymorphism by the PCR-TTGE technique. No pathogenic base substitution was found.

Key Words: MTRNR1, mtDNA mutation, TTGE, aminoglycoside ototoxicity, mitochondrial nonsyndromic deafness,

Savvysavz · Answer

Hi Whiteleaf,

Your essay looks much better now but there are still some corrections/alterations to be made. Since you wish to keep the sentence in green, I will just modify it a little. Give me a while to correct your essay,I will post it back as soon as possible.

Savvy

Savvysavz · Answer

Hi WhiteLeaf,

The two additions you made are very benefical for you but I shifted them in different paragraphs so as to have a proper flow of events. Please check the whole essay and let me know if you need more help. Your essay looks good now though I still have some questions in red. I hope all this fits in one page?

Your thesis made some interesting reading for me.Thanx

Best of luck.

Savvy

____________________________________________________________

I believe that an international PhD position at the Swiss Institute for Experimental Cancer Research-Ecole Polytechnique Fédérale de Lausanne (ISREC-EPFL) corresponds well with my future plans in seeking a distinguished career in the eminent field of genetics. A dissertation in PhD(delete is) provides the cutting edge in the career of a young scientist. I chose “Screening of the mitochondrial 12S rRNA (MTRNR1) gene in patients with sensorineural hearing loss” for my Masters. During my thesis, I screened hundred individuals by PCR-RFLP and PCR-TTGE techniques, for 1555 A>G, and other possible mutations of the MTRNR1 gene. I came out third among the 98 students who graduated from the same department during my academic year of 2002-2003.

Today, a number of people aim to study science. I am one of those who not only aim to achieve a professional scientific understanding but also to use science as a tool in propagating this knowledge for the benefit of humanity. Science will surely help me to contribute a number of things to people and their lives. No education is complete without experience. Armed with a solid academic background, I (delete can) wish to continue my career in PhD which will add to my experience. Since I am already well-versed in theory, I will be able to adapt and modify the theoretical constructs as and when required in order to meet higher practical researches. In addition, discovering the implied meanings behind the scientific knowledge gives me pleasure and encourages me to dwell deeper into the subject matter.

I am not one who constantly studies on one subject alone. I am team spirited and can communicate effectively. I hold strong leadership skills and I am good at organizing (good at organizing what?) and analytical thinking. In many team works or groups, I could lead the team well, and persist till I reach my desired goal. Colleagues from multi-cultural backgrounds from various countries in an international atmosphere can be in my favour. I hold a good social circle of friends as well. Conducting such a graduate study, I first aim to acquire something for myself and then share it with people around me. I like people who do not discriminate others against race, nationality, ***, or religion. I help ailing people (who do not know absolutely) by trying to solve their health problems through my studies in genetics. My future goals include establishing a career in research and educating people about science. As far as history goes, scientific research originates from a very fundamental human character namely curiosity. I believe that curiosity creates questions in a scientist’s mind and from there research starts. My curiosity and strong ambition encouraged me to become a scientist. It is very important, though, to ask the right questions. (delete during my four-year tenure at the university followed by a) During the thesis I conducted at the Molecular Biology Department at Hacettepe University,Pediatric Molecular Genetic Department at Ankara University School of Medicine, I discovered a vital answer on the role that 1555 A>G mutation plays in sensorineural hearing loss from the following question:Which mutations(delete do) induce hearing loss in 12S rRNA (MTRNR1) gene and what are the other changes associated with aminoglycoside ototoxicity? The 1555 A>G mutation was found by PCR-RFLP in one patient with a history of aminoglycoside exposure. Two patients with no history of aminoglycoside exposure were found to have the 750 A>G polymorphism by the PCR-TTGE technique. No pathogenic base substitution was found( delete this sentence in the studies I conducted at the Molecular Biology Department at Hacettepe University, Pediatric Molecular Genetic Department at the Ankara University School of Medicine and) I would like to be one of those who work incessantly to find out more answers. In the future, when I find (delete questions (which I create in my mind)) the right answers to the right questions, I think I will have greater opportunity to become a leader in my field. Keeping the above in mind, I am now applying for your Doctoral Programme in Genetics starting February ***, 2006.

As a committed, thoughtful and hard-working person with a dynamic personality, sound practical and theoretical background, I believe that I make a good candidate for the PhD Program in the field of Genetics in your esteemed Institute.

Note: I am enclosing my Masters thesis abstract for your reference.

ScreenIng of the MitochondrIal 12S rRNA (MTRNR1) Gene in Patients with Sensorineural Hearing Loss

ABSTRACT

DNA damage with accumulation of reactive oxygen species results from mutations causing reduction of Adenosine Triphosphate(ATP) synthesis in mitochondria where the steps of oxidative phosphorylation occur at the final stage of cellular respiration in aerobic organisms. In many tissue and organ systems, this insufficiency results in disorders. Some mutations affect mitochondrial translation, decreasing ATP production in the cochlea of the inner ear. A decreasing amount of ATP (delete affects)alters ion pumping negatively, damaging ion balance, which is very important in normal hearing function. Identified mutations, which are responsible for nonsyndromic hearing impairment in the mitochondrial DNA occur mostly in the MTRNR1 gene.

Mutations in 12S rRNA, which are frequently maternally transmitted (delete frequently) cause hearing loss. They are usually present in small families(families or clusters?) or(delete as sporadic occurrances) occur sporadically. One of the most common non-syndromic mutations in this gene is 1555 A>G. This mutation alone can induce hearing loss but it is also the most common change (change or gene?) associated with aminoglycoside-induced deafness. Another mutation (delT961Cins(n)) in the 12S rRNA gene has also been identified, associated with aminoglycosidic ototoxicity. Pathogenicity of this mutation (delete has remained questionable) is debatable. A number of other changes in this gene have been implied to be associated with hearing loss.

In this study, 70 individuals with hearing impairment, who do not have mutations in the GJB2 gene, were screened by PCR-RFLP and PCR-TTGE techniques, for 1555 A>G, and other mutations in the MTRNR1 gene. Individuals with hearing loss were coming from small (small or afflicted?)families with other case histories or were sporadic cases. Eleven patients had a history of aminoglycoside exposure. Thirty control individuals with no hearing impairment, and negative family history for deafness were also induced.

The 1555 A>G mutation was found by PCR-RFLP in one patient with a history of aminoglycoside exposure. Two patients with no history of aminoglycoside exposure were found to have the 750 A>G polymorphism by the PCR-TTGE technique. No pathogenic base substitution was found.

Whiteleaf · Answer

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