He means that policemen walk a certain way, and as Lestrade walks this way Holmes was able to identify him by the sound of his footsteps.
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First of all, this dialogue is from "the abominable bride". I am from India and here I am to help you getting rid of the confusion. 'Tread' means footsteps and 'regulation' means the act or process of regulating. Therefore, the line, "The regulation tread is unmistakeable" means that 'the pattern of footsteps cannot go unrecognised'. Lestrade told Sherlock,
Danilunmarkable
No. This is not what he said. He said unmistakable.
Maybe you were confused by that. I certainly was.
CJ
Whoops! I just realized.
Why am I replying to a post from two years ago?
And why are so many others doing the same?