Here's a start: It depends on the meaning of the word, and of course the location of the affix, since a prefix by definition goes on the front. 'Biology'-- 'bios' is the central concept, hence the root, while 'logy' is a suffix meaning 'study, knowlege of'. 'Biorhythm'-- here the rhythm is the central concept (root) and the 'bio' tacks on the idea of life as a prefix.
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