JOB Research Assistant

Skills and Qualifications:
• University degree (BSc. or MSc.) in biological sciences or infectious diseases plus 3 years of related research experience
• Proven expertise in molecular biology and recombinant protein expression/purification methodologies mandatory
• Experience in handling biosafety level 2 pathogens required and only individuals with biosafety training at xxx will be considered
• Established experience in the detection and analysis of protein-protein interactions
• Experience with mouse models for the study of infectious pathogens a plus
• Effective oral and written communication, interpersonal and organizational skills
• Computer and excellent record-keeping skills required
• Ability to work effectively as part of a multi-disciplinary team
• Accuracy and attention to detail
• Ability to prioritize tasks to meet project deadlines



I achieved an MSc in Molecular Biology in September xxx with emphasis on molecular diagnosis and therapeutics. I also took recombinant DNA and Bioinformatics courses which introduced me to gene expression and structure function relationships of proteins. I developed interest in genes expressed under drug pressure as well as conserved epitopes in infectious pathogens enabling to be more knowledgeable in bioinformatics programs. However, Molecular microbiology is my preferred branched because it includes main disease control aspects of most pathogens of public health treat and I enjoy critical and analytical work.

As a research techniques and laboratory analyst as indicated in my resume, I used PCR, Dot blotting, ELISA and SDS-PAGE to identify variations in molecular markers in Plasmodium falciparum , to identify point mutations in genes responsible for sensitive of the parasite to given drugs, to identify, qualify and quantify antibodies to specific antigens and to separate and identify proteins present in a sample. Within these periods I safely collected and analysed samples from patients with infectious diseases. I have definitely benefited from these experiences and I feel much more knowledgeable in biosafety practices.

I use optimised reagents and protocols as well as control measures and obtained optimum results for my reactions while observing all laboratory safety procedures. My ability to follow and up date protocols have enabled me to be disciplined and organised. I have carried out analysis with a range of molecular techniques and I feel confident that I can use other techniques with similar principles such as ELISPOT and Immunohistochemistry detection.

I used PowerPoint, MS word, Excel and other soft ware programs in seminars, debates and course works to make graphs, diagrams, calculations and pictures to discuss, explain and indicate the basis and significance of my work, how it is similar and or different from similar works of other investigators.

I plan, arranged and work around my project meeting tight datelines while comparing my ideas with those of my colleagues and laboratory mates and eager to help whenever possible. I report all observations as it is generated in real time in my laboratory notebook and my progress to my supervisors. This will enable the continuation of similar studies based on my findings as well as the continuation of my work by another person.

I am keenly interested in specialising in disease control research mainly on the patterns of gene expression during various conditions and the structure function changes of the proteins produced by the cell type during these conditions.

The xxxx website has given me a proper insight into the expertise, techniques, focus, approach and the pathogens of xxxx. It is my pleasure to learn more about gene expression and its associated proteins as a molecular basis of disease control amongst a variety of top quality experts. This will enable me to develop myself better both professionally and personally. I therefore, feel confident that the Research Assistant is the right choice for me and I would appreciate the opportunity to work at the Division of Infectious Diseases-xxx .
MY CV is as below

Name

address.

Nationality: xxxxxxx.

E-mail: xxxxxx


Key Skills

Master of Science-Medical Molecular Biology

Sound scientific background with academic and practical knowledge in: molecular biology, malaria genetics and recombinant DNA technology. Ability to initiate diverse research projects and achieve significant results. Ability to motivate multi-disciplinary teams and work on own initiative. Competent in Microsoft Series, Primer design and Bioinformatics software programs. Solid analytical skills with the ability to assimilate information quickly/accurately; Excellent communication, interpersonal and presentation skills- at all levels. Adapt well to challenges and resilient. Fluent in English.


Position of Responsibility

1996-1997

Financial Secretary,xxx.

• Registering students, receiving and recording finances
• Assisting Zoology students with departmental enquires and propositions

Career History

Named and add.

2007 – Present

Laboratory Manager

• Disease diagnosis, personnel training and laboratory management

xxxx

2007-Present ( Part-time)

Teaching Assistant

Responsible for facilitating student studies on Molecular Biology and Immunogenetics, through course work and tutorials.


xxxx

2003-2004

Laboratory Manager

• Disease diagnosis, personnel training and laboratory management

xxxx

2001-2003

Research Technician

Investigated polymorphism in merozoite surface genes of Plasmodium falciparum (P. falciparum) as well as it’s resistance to pyrimethamine-sulfadoxine (P/S) drug:

• Collecting Plasmodium faciparum blood samples from endemic regions
• Collecting patient information using observation and questionnaire
• Planning and arranging experiments to provide specific research data
• Analysing samples
• Analysing and reporting findings
• Taking students through experimental procedures
• Laboratory housekeeping

Research Techniques: Microscopy, DNA Extraction, PCR, GEL Electrophoresis, Dot Blotting, GLP



xxx


1999-1999

Facilitator


Researched the livelihood, activities and needs of people living in rural communities

• Taking subjects through prevention and control of Public Health diseases
• Collecting information on activities of rural communities in xxx
• Preparing preliminary report on findings

Education and Training

school

Course

Research Project:

Researched amino acid variations to the circumsporozoite protein gene (csp) incorporated into the RTS, S/AS02 vaccine against Plasmodium falciparum malaria in a study population

• Designing, planning and arranging research project for specific research data
• Collecting and collating results
• Analysing resulting data by computer
• Writing detailed report on findings
• Presenting results

Research Techniques: PCR, Gel Electrophoresis, Gene Purification, DNA Sequencing, GLP


1992-1997 University of xxx

BSc Zoology 7GPA)

-Insight into Genetics and Developmental Genetics


Researched proteins of the foot muscles of fresh water snails Bulinus globosus and Biomphilaria pfeifferi which aids in the transmission of schistosomiasis

Research Techniques: Mortar &Pestle and SDS_PAGE, GLP


1987-1992 Big xxx

7 GCSE: English (B), Mathematics (B), French (C), Chemistry (A), Biology (B), Human Biology (B), Physics (B)


Computer Skills

• Primer 3
• Microsoft Office, Word and Excel
• PowerPoint
• Bioinformatics and Sequencing programs

ACHIEVEMENTS
I identified a faster and easier technique to determine genetic diversity in a Plasmodium falciparum study population. I also initiated Zoology Departmental Orientation for new students in the University of xxx.

PERSONAL INFORMATION
Having achieved a high standard of education, coupled with practical work experience in a range of environments, I have accumulated diverse skills and knowledge specifically in research. I have the ability to plan, organise and monitor my own performance, relishing every opportunity to develop both personally and professionally. I adapt to ever changing environments with initiative and decisiveness, with a flexible attitude towards my work. I now wish to further utilise my abilities working with an Institution who values and recognises core competencies and can provide challenges to develop both professionally and personally.