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Igorazar Posted 12 years ago
Essay & Composition Writing
Please, help me write introduction correctly.
Use of peptidome analysis of human serum for search markers of colorectal cancer.
According to the International Agency for Research on Cancer IARC colorectal cancer (CRC) is the third most common cancer in men and the second - in women. One of the major problems of monitoring and treatment of CRC is its early diagnosis. Depending on the type and location of the tumor, 50-80% of cases, because of the latent course of this disease, it is diagnosed only III or stage IV, which significantly reduces the chances of successful treatment.. Since the mid 70s, the world's been an active search and introduction of laboratory diagnostic methods based on the determination of tumor markers in the serum of the patient. However, despite all the efforts of the international scientific community, specific and sensitive method for diagnostic screening CRC had still not established. The aim of this work is to identify in the serum of patients with CRC peptide markers of the disease using the latest proteomic approaches.
To work used serum samples of 50 patients with colorectal cancer and 50 healthy donors. To isolate peptides from serum used in series with the magnetic microparticle surface, and a weak cation exchange chromatography, anion exchange microcolumns. The resultant anion exchange column with an elution step with a salt gradient and desalted peptide fractions were analyzed by LC-MS/MS for quadrupole TOF mass spectrometer ABSciexTripleTOF + 5600. The resulting arrays of tandem mass spectra were analyzed using the two search engines: Mascot and X! Tandem. Relative quantification identified serum peptides was performed by SWATH (sequential windows acquisition of all theoretical ion-fragments spectra).
According to the International Agency for Research on Cancer IARC colorectal cancer (CRC) is the third most common cancer in men and the second - in women. One of the major problems of monitoring and treatment of CRC is its early diagnosis. Depending on the type and location of the tumor, 50-80% of cases, because of the latent course of this disease, it is diagnosed only III or stage IV, which significantly reduces the chances of successful treatment.. Since the mid 70s, the world's been an active search and introduction of laboratory diagnostic methods based on the determination of tumor markers in the serum of the patient. However, despite all the efforts of the international scientific community, specific and sensitive method for diagnostic screening CRC had still not established. The aim of this work is to identify in the serum of patients with CRC peptide markers of the disease using the latest proteomic approaches.
To work used serum samples of 50 patients with colorectal cancer and 50 healthy donors. To isolate peptides from serum used in series with the magnetic microparticle surface, and a weak cation exchange chromatography, anion exchange microcolumns. The resultant anion exchange column with an elution step with a salt gradient and desalted peptide fractions were analyzed by LC-MS/MS for quadrupole TOF mass spectrometer ABSciexTripleTOF + 5600. The resulting arrays of tandem mass spectra were analyzed using the two search engines: Mascot and X! Tandem. Relative quantification identified serum peptides was performed by SWATH (sequential windows acquisition of all theoretical ion-fragments spectra).
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please, see my introduction. where is mistakes?
- please, see my introduction.
- where is mistakes?
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Use of peptidome analysis of human serum for search markers of colorectal cancer.
According to the International Agency for Research on Cancer, IARC colorectal cancer (CRC), is the third most common cancer in men, and the second in women. One of the major problems of monitoring and the treatment of CRC is early diagnosis. Depending on the type and location of the tumor, 50-80% of cases
According to the International Agency for Research on Cancer, IARC colorectal cancer (CRC), is the third most common cancer in men, and the second in women. One of the major problems of monitoring and the treatment of CRC is early diagnosis. Depending on the type and location of the tumor, 50-80% of cases
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