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Does "DIAGNOSIS AND CHARACTERIZATION OF PATHOGENS" mean "DIAGNOSIS OF PATHOGENS AND CHARACTERIZATION OF PATHOGENS"?

Context:

DIAGNOSIS AND CHARACTERIZATION OF PATHOGENS

In the late 1800s, the realization that identifiable microbes caused specific diseases led to pathogen-specific medical diagnosis. Although the time-honored techniques of growing bacteria in broth or solid cultures and staining and examining them under microscopes are still important today, newer technologies have transformed the field of microbial diagnosis. Among the first emerging epidemic diseases to be identified by one such method was the hantavirus pulmonary syndrome, a centuries-old disease caused by an unknown phlebovirus (Sin Nombre) that was discovered unexpectedly in 199350 by the application of a then-novel molecular genetic technique, polymerase chain reaction (PCR). This followed quickly on the 1992 discovery of the previously unknown agent causing an infectious chronic condition, Whipple's disease.51 Less than a year later, PCR-related subtraction techniques solved a century-old mystery of the cause of Kaposi's sarcoma, human herpesvirus 8.52Now, less than two decades later, sophisticated, high-throughput, rapid sequencing of the genomes of pathogens not only dramatically hastens initial identification but also detects individual genetic variants,53 facilitating identification of the genetic basis of drug resistance. Additional gene-based diagnostic tools include microchips and other technologies that detect short sequences of many different genes or their proteins, allowing simultaneous diagnosis or diagnostic elimination of multiple pathogens. New serologic techniques such as enzyme-linked immunosorbent assay can be many times more sensitive than traditional techniques in detecting and measuring antibodies to pathogens. Furthermore, monoclonal antibody techniques, which involve the use of cellular clones to produce antibodies against specific pathogen epitopes, have been adapted for the purposes of diagnosis, identification of the molecular structures of pathogens, elucidation of the natural history and pathogenesis of infectious diseases, development of conformationally accurate immunogens to be used as vaccine candidates,54 and even treatment.55 Many of these data-rich approaches require sophisticated bioinformatics systems (e.g., phylogenetic comparisons and genome construction analyses).