Defying culture in the laboratory?

Does "defying culture in the laboratory"negating lab culture/lab rules"?

Context:

This research was conducted under the DOE JGI's continuing effort to explore the biological frontier known as "microbial dark matter." These are the vast number of microbes that are difficult-to-impossible to grow and study in the laboratory but populate nearly all environments from the human gut to the hot vents at the bottom of the ocean. Approximately 99% of all microbial species on Earth fall in this category, defying culture in the laboratory but profoundly influencing the most significant environmental processes from plant growth and health, to the carbon and other nutrient cycles on land and sea, and even climate processes

http://www.sciencedaily.com/releases/2014/05/140522141422.htm