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Jayyyem Posted 16 years ago
Essay & Composition Writing

Can someone please help me reword this for my lab report? thank you :)

In the field of biology, there are many prevalent subdivisions and areas of study that have been found relatively recently. One of these is Biotechnology, the area of study where technological applications along with biological knowledge leads to a wide array of possible applications, from genomic alterations for transposing genes between species to specific bodily-essential substances such as insulin or lactase[2][3 ]. However the most prevalent of this delves into the field of immunology and pathology, were biotechnology allows for induction of resistance to certain agent through usage of plasmids [1][2][6 ]. Plasmids, which are extra chromosomal DNA strands which can be manipulated to input new genetic material, such as resistance-carrying genes and allowing this material to be input into the host cell's nucleus for replication of the altered gene into future offspring, granting resistance to them as well[1][2][4][5][6 ].
In the scenario of this experiment, E.coli, a fast reproducing prokaryotic bacterial cell will be granted ampicillin resistance via plasmid alteration, inputting B-lactamase enzyme precursor which will grant future e.coli offspring from that strand the ability to produce B-lactamase[1][5][6 ]. B-lacatamase cleaves B-lactam, the active factor in antibiotics which is a nitrogenous-like ring in the center of most antibiotics molecule[2][3][5][6 ]. The B-lactam ring in ampicillin and other antibiotics such as penicillin, erythromycin and amoxicillin, among many others, causes the degradation of the cell wall by destroying cross-bridges in the periplasm of cell wall, thus causing the cell wall of the bacterial agent to collapse[3][5][6 ].
The experiment will evaluate whether the input of antibiotic resistance grants cells resiliency against ampicillin (pAMP), thus enabling them to survive[1][6 ]. Additional factors being applied to the e.coli strands are procedures such as "heat shocking" and exposure to Calcium Chloride[1][5][6 ]. These processes allow the cell to become more competent by increasing their affinity for the external plasmid DNA to come into the nucleus by making it positively charged and making the nucleus more porous which allows a greater intake of plasmid to enter the nucleus for a greater degree of transformation efficiency[1][2][6 ].
Another two samples will be prepard with e.coli but will be exposed neither ampicillin or pAMP, preferably treated as control groups[1 ]. These dishes will be used to show a contrast of unhampered bacterial growth against growth in unfavorable conditions[1 ].
  

Top answer

Hi, Why do you want to reword it? Clive

  • Hi, Why do you want to reword it?
  • Clive
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1 Answers
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Hi,

Why do you want to reword it?

Clive

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