The author presumably just wanted to make a more visible separation between "by herself" and "afterwards", both of which modify "she took a breath". The comma is not mandatory.
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moominpapaSo, which is to say, if there were not the comma, it might be misunderstood as another meaning or having another nuance?No, I don't really think so.
moominpapaOh, or the visible separation you said is to emphasize each two phrases: "by herself" and "afterwards"?Possibly it has a mild effect of that sort, but it is not a big deal.
Emily Hill no you cant do thatI think you mean "No, you can't do that."
Ok, I got it! Thank you so much!
Possibly it has a mild effect of that sort, but it is not a big deal.